报告题目：A novel mechanism of small RNA biogenesis in a causative protozoan- a data driven model generation

报  告 人： 张力烨 博士

Boston University School of Medicine

Department of Medicine, Computational Biomedicine

报告时间：7月9日，14:00-15:00

报告地点：闵行校区生物药学楼2#123

联  系 人：张晓君 This e-mail address is being protected from spambots. You need JavaScript enabled to view it.

 

Abstracts:Small RNA biogenesis (such as microRNA and siRNA) typically involves cleavage of structured precursors by RNase III-like endonucleases. However, guide RNAs that direct U-insertion/deletion mRNA editing in mitochondria of trypanosomes maintain 5′ triphosphate characteristic of transcription start site and possess U-tail indicative of 3′ processing and uridylation. Here, we will show you how computational analysis integrates multiple RNA-Seq assays (Stranded RNA-Seq, CLIP-Seq and RNA-IP-Seq) and guides biochemistry experiments and then lead to a completely novel model for guide RNA generation in which gRNA is derived from the uridylation-induced, antisense transcription-controlled exonucleolytic degradation.

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